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A new technique for physiodesis using photodynamic therapy.

Stuart K Bisland1, Crystal Johnson, Mohamed Diab

  • 1Princess Margaret Hospital, Department of Medical Physics, Uniersity Health Network, Toronto, Ontario, Canada. sbisland@uhnres.utoronto.ca

Clinical Orthopaedics and Related Research
|April 7, 2007
PubMed
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Photodynamic therapy can induce premature vascularization and calcification in mouse growth plates. This technique shows potential for limb length correction, offering a novel approach to physiodesis.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Skeletal Biology

Background:

  • Vascularization of growth plates is crucial for endochondral ossification.
  • Controlling growth plate vascularization could offer therapeutic interventions for skeletal disorders.

Purpose of the Study:

  • To investigate the potential of photodynamic therapy (PDT) to induce premature vascularization and calcification in mouse growth plates.
  • To assess the efficacy of PDT in altering growth plate dynamics and subsequent limb length.

Main Methods:

  • Utilized transgenic mice with vascular endothelial growth factor (VEGF) gene-induced bioluminescence.
  • Administered benzoporphyrin derivative mono-acid-mediated PDT to the proximal tibial growth plate.
  • Quantified VEGF bioluminescence, VEGF protein expression, CD31-labeled vasculature, and limb length via radiography.

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Main Results:

  • Repeat PDT treatments (10 J x2) enhanced VEGF bioluminescence within 2 weeks.
  • Histology revealed increased vasculature near growth plates and evidence of physeal closure.
  • Limb shortening averaged 9.5% at 4 weeks post-treatment without complications.

Conclusions:

  • Photodynamic therapy can successfully induce premature vascularization and calcification in growth plates.
  • PDT demonstrates potential as a method for achieving physiodesis and correcting limb length discrepancies.