Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 15, 2026

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
06:28

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Published on: February 17, 2023

Cell microvesicles during experimental endotoxemia.

L D Zubairova1, D M Zubairov, I A Andrushko

  • 1Department of Biochemistry, Kazan State Medical University. dilja2001@mail.ru

Bulletin of Experimental Biology and Medicine
|April 7, 2007
PubMed
Summary

Endotoxin administration in rabbits induced microvesicle formation in arterial blood, impacting blood clotting and red blood cell hemolysis. These findings shed light on microvesicle dynamics during the generalized Shwartzman phenomenon.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[MICROBIAL COMPOSITION OF VARIOUS SURFACES OF SKIN DURING DEVELOP- MENT OF ATOPIC DERMATITIS BASED ON DATA FROM MALDI-TOF MASS-SPECTROMETRY IDENTIFICATION METHOD].

Zhurnal mikrobiologii, epidemiologii i immunobiologii·2016
Same author

[THROMBIN-MEDIATED EFFECTS OF BLOOD MICROPARTICLES ON FORMATION, STRUCTURE, AND STABILITY OF FIBRIN CLOTS].

Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova·2015
Same author

Effect of blood microparticles on the kinetics of polymerization and enzymatic hydrolysis of fibrin.

Doklady. Biochemistry and biophysics·2015
Same author

[3-Hydroxy-1,5-diaryl-4-pivaloyl-2,5-dihydro-2-pyrrolones induce the mitotic exit failure and cell death in tumor cells in vitro].

Tsitologiia·2015
Same author

Effects of Staphylococcus aureus supernatants on human lymphocyte surface receptors.

Bulletin of experimental biology and medicine·2013
Same author

Lymph and blood cytokines in fever of different severity.

Bulletin of experimental biology and medicine·2012

Area of Science:

  • Vascular Biology
  • Hematology
  • Immunology

Background:

  • The generalized Shwartzman phenomenon is a severe systemic inflammatory response.
  • Microvesicles play a role in various physiological and pathological processes.
  • Understanding microvesicle dynamics is crucial for studying thrombotic events.

Purpose of the Study:

  • To investigate the formation and characteristics of microvesicles in arterial blood.
  • To analyze the impact of endotoxin on microvesicle content and composition.
  • To observe changes in blood clotting and erythrocyte integrity.

Main Methods:

  • Induction of generalized Shwartzman phenomenon in rabbits via sequential endotoxin injections.
  • Quantification of microvesicles in arterial blood.

More Related Videos

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
08:27

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

Related Experiment Videos

Last Updated: Jul 15, 2026

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
06:28

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Published on: February 17, 2023

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
08:27

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

  • Analysis of ecto-5'-nucleotidase presence on microvesicles.
  • Measurement of arterial blood clotting time.
  • Assessment of erythrocyte hemolysis.

    • Main Results:

    • Intravenous endotoxin injections led to increased microvesicle content in arterial blood.
    • A subset of these microvesicles were found to contain ecto-5'-nucleotidase.
    • Biphasic alterations were observed in arterial blood clotting time.
    • Erythrocyte hemolysis showed biphasic changes.

    Conclusions:

    • Endotoxin challenge triggers significant microvesicle release into arterial circulation.
    • Microvesicles, some expressing ecto-5'-nucleotidase, are implicated in the pathogenesis of the generalized Shwartzman phenomenon.
    • The observed changes in clotting time and hemolysis suggest a complex role for microvesicles in endotoxemia-induced vascular injury.