Related Experiment Video

Updated: Jul 15, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Expression of major vault protein gene in osteosarcoma patients

Cristiane Arruda Dalla-Torre1, Silvia Regina Caminada de Toledo, Maisa Yoshimoto

  • 1Department of Pediatrics, Instituto de Oncologia Pediátrica, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil, and Applied Molecular Oncology, Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Ontario, Canada.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|April 7, 2007
PubMed

Insights

Major vault protein (MVP) expression in osteosarcoma (OS) tumors correlates with metastasis and poorer event-free survival (EFS) after chemotherapy, suggesting MVP is involved in drug resistance mechanisms.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is a primary bone cancer with significant treatment resistance.
  • Over 30% of OS tumors are resistant to current chemotherapeutic agents.
  • Understanding drug resistance mechanisms is crucial for improving patient outcomes.

Purpose of the Study:

  • To screen for genes involved in drug resistance in osteosarcoma.
  • To investigate the role of Major Vault Protein (MVP) in OS chemoresistance.
  • To correlate MVP expression with clinical and pathological features.

Main Methods:

  • Utilized GEArray Q Series Human Cancer Drug Resistance and Metabolism Gene Array for gene screening.
  • Validated MVP gene expression using real-time PCR on 45 OS patient tumor samples.
  • Correlated MVP expression with clinical data, including metastatic status and event-free survival (EFS).

Main Results:

  • MVP expression was detected in 53% of OS tumor samples.
  • MVP expression correlated with metastatic disease at diagnosis after neoadjuvant chemotherapy (p=0.048).
  • MVP expression was associated with worse event-free survival (EFS) (p=0.036).

Conclusions:

  • MVP expression is implicated as a mechanism of drug resistance in osteosarcoma.
  • Chemotherapy appears to induce MVP expression in OS.
  • MVP may serve as a potential biomarker for predicting treatment response and prognosis in OS patients.

More Related Videos

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Methods to Enable Spatial Transcriptomics of Bone Tissues07:43

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

Related Concept Videos

Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...