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Updated: Oct 17, 2025

The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
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Western blotting

Choogon Lee1

  • 1Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2007
PubMed

Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane. A specific antigen immobilized on the membrane is detected and visualized by a primary antibody, a secondary antibody-peroxidase conjugate, and a chemiluminescent reagent.

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