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Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

Published on: January 30, 2013

RNA in situ hybridizations on Drosophila whole mounts

Corinna Wülbeck1, Charlotte Helfrich-Förster

  • 1Institut für Zoologie, Universität Regensburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2007
PubMed

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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...