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Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Published on: January 23, 2012
A genomic integration method to visualize localization of endogenous mRNAs in living yeast
Liora Haim1, Gadi Zipor, Stella Aronov
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Nature Methods
|April 10, 2007
Abstract:
mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3' untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).

