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Published on: August 28, 2018
Correlation between HSP-72 expression and IL-8 secretion in human mesothelial cells
T O Bender1, A Riesenhuber, M Endemann
1Department of Nephrology and Medical Intensive Care, Universitätsklinikum Charité, Campus Virchow-Klinikum, Berlin, Germany.
The International Journal of Artificial Organs
|April 10, 2007
Summary
Peritoneal dialysis fluid (PDF) exposure increases heat shock protein 72 (HSP-72) and interleukin-8 (IL-8) in mesothelial cells, indicating a stress response linked to inflammation and potential membrane failure.
Area of Science:
- Nephrology
- Cell Biology
- Biochemistry
Background:
- Peritoneal dialysis fluid (PDF) cytotoxicity and inflammation cause chronic mesothelial injury and peritoneal membrane failure.
- Heat shock protein 72 (HSP-72) indicates cellular stress response.
- Interleukin-8 (IL-8) is a marker for pro-inflammatory cytokines.
Purpose of the Study:
- To correlate HSP-72 induction with IL-8 secretion in mesothelial cells after sublethal PDF exposure.
- To investigate the cellular stress response to PDF in peritoneal dialysis.
Main Methods:
- Primary human mesothelial cells were exposed to sublethal PDF.
- HSP-72 expression and IL-8 secretion were measured after a 24-hour recovery period.
- Immunodensitometry and ELISA were used for assessment.
Main Results:
- PDF exposure increased HSP-72 expression and IL-8 secretion in a time-dependent manner.
- HSP-72 levels rose significantly with PDF exposure (p<0.05).
- IL-8 secretion increased significantly with PDF exposure (p<0.05), with no impact on cell viability.
Conclusions:
- A significant correlation exists between HSP-72 expression and IL-8 secretion.
- This suggests mesothelial cell pro-inflammatory pathways are part of the stress response to PDF.
- Further research into cellular regulatory mechanisms is warranted.
