Related Experiment Video
Updated: Jul 15, 2026

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
Published on: March 24, 2010
The pattern and evolution of yeast promoter bendability
Itay Tirosh1, Judith Berman, Naama Barkai
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Abstract:
In Saccharomyces cerevisiae, transcription factor binding sites are found preferentially approximately 100-200 bp upstream of the start codon. Here, we show that this region is associated with rigid DNA in promoters lacking a TATA box, but not in TATA-containing promoters. The association of rigid DNA with transcription factor binding sites is conserved in TATA-less promoters from 11 yeast species, whereas the position of the rigid DNA varies substantially among species. Rigid DNA could influence nucleosome positioning and assist in the assembly of the transcriptional machinery at TATA-less promoters.
Related Concept Videos
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Yeast Signaling
Bioreactor Controls-III
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial Transcription
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:

