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Erythrina lectins detect the H/HI blood groups
D Sudakevitz1, N Gilboa-Garber, C Levene
1Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|August 1, 1991
Summary
Erythrina lectins from seeds exhibit specific ABO(H) blood group activity, primarily interacting with the H antigen and showing strongest hemagglutination with O(H) erythrocytes. These lectins prefer H/HI blood groups but are not inhibited by secretions.
Area of Science:
- * Glycobiology and immunology
- * Lectin biochemistry and characterization
Background:
- * Lectins are proteins with specific carbohydrate-binding properties.
- * ABO(H) blood group system is crucial in transfusion medicine and diagnostics.
- * Erythrina lectins are plant-derived proteins with potential diagnostic applications.
Purpose of the Study:
- * To determine the ABO(H) blood group specificity of Erythrina corallodendron lectin.
- * To compare the reactivity of Erythrina corallodendron and Erythrina indica lectins with various blood group antigens.
- * To investigate the interaction of these lectins with H and I/i antigens.
Main Methods:
- * Purification of lectin from Erythrina corallodendron seeds.
- * Hemagglutination assays using erythrocytes from different ABO(H) and Ii blood groups.
- * Inhibition assays with saliva from ABO(H) secretors.
Main Results:
- * Erythrina corallodendron lectin showed strongest hemagglutination with O(H) and weakest with Oh(Bombay) red blood cells.
- * Lectins demonstrated preference for O(H)I > A2I > O(H)i adult > A2BI > BI > O(H)i cord > A1I > A1i adult > Bi cord > A1BI > Ai cord > ABi cord > OhI.
- * Both lectins exhibited H/HI blood group preference but were not inhibited by ABO(H) secretor saliva.
Conclusions:
- * Erythrina lectins bind preferentially to H/HI blood group antigens.
- * These lectins can be classified with other lactose-inhibited lectins, distinct from those inhibited by H substances in secretions.
- * Potential for use in blood group typing and immunological studies.