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Published on: June 1, 2012
[Effect of calcium on reconstruction of basement membrane in composite chitosan tissue engineering skin]
Yuangang Lu1, Jinjin Wu, Xia Lei
1Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing, 400042, PR China.
Objective:
To search the most suitable concentration of calcium in the medium for the basement membrane reconstruction in tissue engineering skin in vitro.
Methods:
Composite chitosan tissue engineering skin was prepared according to previous studies. Four groups were included according to the concentration of calcium (1.00, 1.45, 1.65 and 1.95 mmol/L respectively). After 7 days and 15 days of culture, the histological manifestation of basement membrane in tissue engineering skin was observed by hematoxylin & eosin staining and PAS staining, and collagen IV of basement membrane was detected immunohistochemically at the dermal-epidermal junction.
Results:
This tissue engineering skin shared some histological features of normal skin, including a well-differentiated stratified epidermis and a dense dermis. The epithelium in the group of 1.95 mmol/L. Llcium differentiated better than those in other groups. PAS staining showing a regularly red dying strap domain at the dermal-epidermal junction. Collagen IV was positively stained immunohistochemically at the dermal-epidermal junction in the tissue engineering skin.
Conclusion:
The above results suggest that the medium with 1.95 mmol/L calcium should be suitable for the growth of composite chitosan tissue engineering skin and the reconstruction of basement membrane.
