Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimizing gene suppression in schistosomes using RNA interference.

Greice Krautz-Peterson1, Magdalena Radwanska, David Ndegwa

  • 1Molecular Helminthology Laboratory, Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University, Cummings School of Veterinary Medicine, Grafton, MA 01536, USA.

Molecular and Biochemical Parasitology
|April 11, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protection of Mice Orally Infected With Shiga Toxin-Producing <i>Citrobacter rodentium</i> Treated With Nanobody Multimer.

Open forum infectious diseases·2026
Same author

Comprehensive Mapping of Immune Nanobody Repertoires with NanoMAP.

bioRxiv : the preprint server for biology·2026
Same author

Thiamine (Vitamin B1) metabolism in schistosomes.

Biochimie·2026
Same author

Development of a camelid single-domain antibody-based antigen detection assay for the pan-specific diagnosis of active human and animal <i>Trypanosoma brucei</i> infections.

Journal of clinical microbiology·2025
Same author

Generation of Immune Modulating Small Metabolites-Metabokines-By Adult Schistosomes.

Pathogens (Basel, Switzerland)·2025
Same author

<i>In-vivo</i>-targeted gene delivery using adenovirus-antibody site-specific covalent conjugates.

Molecular therapy. Methods & clinical development·2025

Optimized RNA interference (RNAi) protocols effectively suppress Schistosoma mansoni gene expression in parasitic stages. Electroporation delivery of double-stranded RNA (dsRNA) proved highly efficient for targeting cathepsin B.

Area of Science:

  • Parasitology
  • Molecular Biology
  • Public Health

Background:

  • Schistosomes are parasitic platyhelminths causing significant global health issues.
  • Gene expression control is crucial for understanding and combating schistosome infections.
  • RNA interference (RNAi) offers a potential tool for functional genomics in schistosomes.

Purpose of the Study:

  • To develop and optimize protocols for gene silencing in intra-mammalian schistosome stages using RNAi.
  • To evaluate different delivery methods and RNAi triggers for effective gene suppression.
  • To assess the susceptibility of various schistosome life stages to RNAi.

Main Methods:

  • Developed optimized RNA interference (RNAi) protocols for Schistosoma mansoni.
  • Utilized double-stranded RNA (dsRNA) targeting the cathepsin B gene (SmCB1).

Related Experiment Videos

  • Compared electroporation versus soaking for dsRNA delivery; assessed long dsRNAs and siRNAs; used fluorescein-labeled dsRNA for uptake studies.

    • Main Results:

    • Electroporation was 100-1000 times more efficient than soaking for dsRNA delivery.
    • Both long dsRNAs and synthetic short interfering RNAs (siRNAs) effectively suppressed gene expression.
    • Gene suppression lasted up to 40 days, indicating potential amplification of the RNAi process.

    Conclusions:

    • Optimized RNAi protocols, particularly with electroporation, enable effective gene suppression in Schistosoma mansoni.
    • Susceptibility to RNAi varies with parasite life stage and dsRNA uptake routes.
    • RNAi is a powerful tool for functional studies and potential therapeutic strategies against schistosomiasis.