The circular dichroism of tumor necrosis factor-alpha: measurement into the vacuum UV and analysis for secondary structure
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J H Riazance-Lawrence1, A Toumadje, W C Johnson
1Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Chirality
|January 11, 1991
Abstract:
The circular dichroism (CD) spectrum of tumor necrosis factor-alpha has been measured into the vacuum UV to 168 nm. Analysis of the CD for secondary structure is in good agreement with X-ray diffraction results, but the analysis is somewhat unstable. Adding the CD of this protein together with its X-ray determined secondary structure to the basis set should improve subsequent analyses of CD spectra for other all-beta proteins.