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Related Experiment Videos

Controlled and modulated release of basic fibroblast growth factor.

E R Edelman1, E Mathiowitz, R Langer

  • 1Biomedical Engineering Center, Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139.

Biomaterials
|September 1, 1991
PubMed
Summary

Controlled release of basic fibroblast growth factor (bFGF) is crucial for tissue repair studies. Stabilizing bFGF with heparin-Sepharose beads and encapsulating it in alginate microspheres preserves its activity for sustained delivery.

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Area of Science:

  • Biotechnology
  • Biomaterials Science
  • Cell Biology

Background:

  • Basic fibroblast growth factor (bFGF) is vital for cell growth and tissue repair.
  • Its instability limits pathophysiologic studies, as it degrades rapidly after administration.
  • Previous controlled-release methods using polymer matrices resulted in significant loss of bFGF activity.

Purpose of the Study:

  • To develop a stable, controlled-release system for basic fibroblast growth factor (bFGF).
  • To preserve the mitogenic activity of bFGF during sustained release.
  • To investigate the chronic effects of bFGF through controlled delivery.

Main Methods:

  • Basic fibroblast growth factor (bFGF) was stabilized by binding to heparin-Sepharose beads.
  • The stabilized bFGF was encapsulated within alginate microspheres for controlled release.

Related Experiment Videos

  • Release kinetics were studied, and biological activity was assessed using BALBc/3T3 fibroblasts.
  • Enzymatic cleavage with heparinase was used to activate and regulate bFGF release.

    • Main Results:

    • Stabilization with heparin-Sepharose allowed for prolonged storage and handling of bFGF.
    • Alginate microspheres achieved 77% encapsulation efficiency.
    • The system released 87.5% +/- 12% of bFGF in a biologically active form.
    • Controlled release profiles were established, and effects on fibroblasts were examined.

    Conclusions:

    • Heparin-binding stabilizes basic fibroblast growth factor (bFGF), preserving its activity in controlled-release systems.
    • Alginate microspheres provide an effective matrix for sustained and biologically active delivery of bFGF.
    • This method enables future studies on the chronic effects of bFGF in tissue repair and other applications.