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Updated: Jul 15, 2026

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
Published on: September 21, 2017
[Effect of dimethoate on dopamines neurotransmitters in brain regions of rat]
Guohong Ruan1, Qiang'en Wu, Qing Wu
1School of Public Health, Fudan University, Key Lab. of Public Health Safety of Education Ministry, Shanghai 200032, China.
Objective:
To observe changes of dopamine and its metabolite (3,4-hydroxyphenylaceticacid, DOPAC) levels of the brain regions in rats exposed by dimethoate (DM).
Methods:
Groups of rats were treated with saline and 38.9, 83.7 and 180.0 mg/kg DM respectively, and were decapitated at different time course from 0.5 to 24 hours after the administration. The dopamine neurotransmitters and its metabolite were determined by reverse-phase high-performance liquid chromatography with electrochemical detection.
Results:
The concentrations of DA (28% - 122% of control) and DOPAC (89% - 538% of control) varied according with the DM dose and the exposure time.
Conclusion:
These results indicated that DM-induced toxic effects could alter the dopamine levels after the different dose and time exposure in the brain regions of rats.
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