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Updated: Jul 15, 2026

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Published on: October 10, 2020
[Experssion of human glutathione-S-transferase A1 in CHO cells]
Pingping Chen1, Qi Wang, Wenjie Li
1Department of Nutrition and Food Hygiene, Public Health College, Zhengzhou University, Zhengzhou 450052, China. chenpingping871@sohu.com
Objective:
To establish the Chinese Hamster Ovary (CHO) expression system for the human glutathione-S-transferase (GST) A1.
Methods:
CHO cells was transfected with recombinant vector pDNA3.1- GSTA1 by lipofectamineTM 2000. The expression of selected CHO-GSTA1 in G418-resistant clones were determined by PCR, RT-PCR and Western blot. CHO-GSTA1 high-expressed clone was cultured to collect supernatant and activity was assayed by U-2001 ultraviolet spectrophotometer.
Results:
CHO-GSTA1 was obtained by PCR, RT-PCR and Western blot. The expressed protein of GSTA1 had biological activity, it was 35 mmol/(mg min pro).
Conclusion:
The CHO-GSTA1 was constructed and for research purification protein and candidate strains of detoxicitic mechanism of chemical toxins and carcinogenesis.
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