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Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
[Expression and identification of Cry 1Ie in Bacillus coli]
Haibin Xu1, Xiaoxia Zhang, Fang Li
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing.
Objective:
In this study, Cry1Ie expressed protein in the transgenic Cry1Ie maize was used as a model protein to establish a technical platform for the safety assessment of other genetically modified plants.
Methods:
Bacillus coli expression system was used to express Cry1Ie protein and then the expressed protein was purified by SDS-PAGE. The equivalence of Cry1Ie proteins expressed in Bacillus coli and in transgenic Cry1Ie maize was analyzed by their immunorecognition and bioactivity.
Results:
Cry1Ie protein was highly expressed in Bacillus coli and could be segregated and purified by SDS-PAGE. Cry1Ie expressed protein in Bacillus coli is substantially equivalent to that of maize genetically modified with Cry1Ie in tests of immunorecognition western blot and bioactivity in target pest.
Conclusion:
The established technical platform of external Cry1Ie expressed protein can be applied to assess the food safety of genetically modified plants.
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