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Updated: Jul 15, 2026
Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
Published on: April 22, 2011
[Study on inducing differentiation of mouse embryonic stem cells into cardiomyocyte in vitro]
Zhou Yu1, Haibin Xu, Weixing Yan
1Institute of Nutrition and Food Safety, Chinese CDC, Beijing 100021, China.
Objective:
To study the differentiation from embeyonic stem cells into cardiomyocyte.
Methods:
Gene alpha/ beta-MHC, a specific gene of cardiomyocyte and another three house-keeping genes were detected by RT-PCR from the ESCs of 0-,2-,4-,6-,8-, 10-,12-,14-,16-,18- and 20 day and ESCs of 0-,3-, 5- and 10 day with specific culture condition.
Results:
ESCs started to express alpha/betaMHC gene from 6 day and the levels of alpha/betaMHC increased with culture time. The levels of 14 day's alpha/betaMHC were the highest. After 14 day, alpha/betaMHC gene kept the stable levels. The levels of three house-keeping genes changed significantly with culture time, and GAPDH kept the best stability of three genes.
Conclusion:
The cycle of the differentiation from ESCs into cardiomyocyte in vitro could be designed as 10 - 12 days and GAPDH could be selected as the fittest inner comparison.
