Related Experiment Video
Updated: Jun 14, 2026
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
[Study on the comparison of rDNA and ITS sequence of Fumonisin-producing strains]
1Institute of Nutrition and Food safety, Chinese Center for Disease Control and Food Safety, Beijing 100050, China.
Objective:
To study the phylogenetic relationships of fumonisin-producing Fusarium verticillioides isolates from China and Gibberella fujikuroi species complex.
Methods:
5.8S, 28S rDNA and ITS1 and ITS2 sequences of 4 ATCC typical strains and 12 isolates from different geographical regions and different samples in China and other countries were determined and analyzed. The results were submitted to GenBank. Phylogenetic tree and homology tree were illustrated. The characteristics of etiological distribution of toxigenetic Fusarium verticillioides and the relativity of of rDNA ITS2 sequence and the fumS gene was elucidated by comparing the isolates from China, South Africa and USA.
Results:
It was found that rDNA highly devergent ITS2 sequences of our isolates with positive fumonisin biosynthase gene were type I, which was consistant with fumonisin-producing isolates from South Africa. The Fusarium verticillioides isolates with ITS2 type II sequence were fumS gene negative. Two Fusarium subglutinans isolates SD-fmv047 and SD-fmv049 were nonfumonisin-producing and ITS2 sequences were neither type I nor II. The DNA sequences had high homology with Fusarium oxysporum.
Conclusion:
New and reliable theory and technology supports were provided for the further study of phylogenetic relationships of Gibberella fujikuroi species complex.
More Related Videos
09:44RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
Published on: December 21, 2015
11:48A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
Published on: October 28, 2021