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Updated: Jul 15, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
Published on: August 28, 2021
Promise of multiphoton detection in discovery and diagnostic proteomics
Jasminka Godovac-Zimmermann1, Claire Mulvey, Maria Konstantoulaki
1University College London, Department of Medicine, Centre for Molecular Medicine, London, UK. j.godovac-zimmermann@ucl.ac.uk
Abstract:
Proteomics has lacked adequate methods for handling the complexity (hundreds of thousands of different proteins) and range of protein concentrations (> or =10(6)) of eukaryotic proteomes. New multiphoton-detection methods for ultrasensitive detection of proteins produce 10,000-fold gains in sensitivity and allow highly quantitative, linear detection of 50 zmol (30,000 molecules) to 500 fmol of proteins in complex samples. The potential of multiphoton detection in top-down proteomics analyses is illustrated with applications in monitoring proteomes in very small numbers of cells, in identifying and monitoring complex functional isoforms of cancer-related proteins, and in super-sensitive immunoassays of serum proteins for high-performance detection of cancer.
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