Approaches to the study of the cell secretome

Yetrib Hathout1

  • 1Children's National Medical Center, Center for Genetic Medicine, Washington, DC 20010, USA. yhathout@cnmcresearch.org

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Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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