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Updated: Jul 15, 2026
Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation
Published on: June 14, 2024
[Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese
Ming-xin Pan1, Fan-dong Kong, Zhi-feng Wu
1Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Objective:
To investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs.
Methods:
Twenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer. Histopathological assessment was carried out to identify the hepatocyte injury using optical and transmission electron microscopy.
Results:
Ischemia preconditioning resulted in a notable increase in HSP70 expression and milder injury of the hepatocyte microstructure, whereas ischemia-reperfusion caused a significant increase of serum transaminases level (P<0.01) with declined HSP70 expression and obvious microstructural changes of the liver tissue.
Conclusion:
Ischemic preconditioning can produce obvious protective effects on the donor liver, and positively regulates the expression of shock protein.
