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Updated: Jan 12, 2026
Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
Published on: January 8, 2018
[Fusion expression of human renal cell carcinoma-associated antigen G250/MN/CA IX in prokaryotic expression system]
Yao-dong Jiang1, Shao-bin Zheng, Wang-long Tan
1Department of Urology, Southern Medical University, Guangzhou 510515, China. jyd@fimmu.com
Objective:
To achieve high expression of human renal cell carcinoma-associated antigen G250 in Escherichia coli.
Methods:
The gene fragments encoding the protein obtained by PCR was cloned into prokaryotic expression vector pET32a(+) and expressed in E. coli Rosseta. The immunogenicity of the recombinant protein was evaluated by Western blotting.
Results:
The plasmid pET32a(+)/G250 was constructed and expressed in E. coli Rosseta successfully. Western blot analysis showed that the recombinant protein could be specifically recognized by monoclonal antibody M75.
Conclusion:
Efficient G250 expression is achieved in prokaryotic expression system, which may facilitate further functional study of the protein and its monoclonal antibody preparation.
