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Updated: Jul 15, 2026
10:37
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Published on: December 9, 2016
Affinity precipitation an option for early capture in bioprocessing
Ruth Freitag1, Ivo Schumacher, Frank Hilbrig
1University of Bayreuth, Bayreuth, Germany. bioprozesstechnik@uni-bayreuth.de
Biotechnology Journal
|April 13, 2007
Summary
Affinity precipitation uses stimuli-responsive polymers to isolate target molecules from complex solutions. This bioseparation technique is scalable and compatible with
Area of Science:
- Bioseparation techniques
- Polymer chemistry
- Biotechnology
Background:
- Affinity precipitation is a bioseparation method.
- Stimuli-responsive polymers undergo reversible phase transitions.
- Ligand conjugates capture target molecules.
Purpose of the Study:
- To discuss the development of affinity precipitation procedures.
- To explore instrumentation and scale-up.
- To present applications in isolating proteins and polynucleotides.
Main Methods:
- Coupling affinity ligands to stimuli-responsive polymers.
- Utilizing reversible polymer precipitation for molecule isolation.
- Applying the technique to complex biological solutions.
Main Results:
- Demonstrated the co-precipitation and isolation of target molecules.
- Showcased compatibility with 'discardibles only' downstream processing.
- Confirmed scalability over several orders of magnitude.
Conclusions:
- Affinity precipitation is an effective bioseparation technique.
- The method is versatile for isolating proteins and polynucleotides.
- The approach offers scalability and process simplification.
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