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Related Experiment Video

Updated: Jul 15, 2026

Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells

Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells

Published on: May 15, 2017

Structural and functional differences of SWIRM domain subtypes.

Misao Yoneyama¹, Naoya Tochio, Takashi Umehara

¹RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.

Journal of Molecular Biology

|April 13, 2007

Summary

The SWIRM domain in MYSM1 protein has a structure similar to Swi3, but lacks DNA binding. This reveals functional divergence within the SWIRM protein family.

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Published on: November 25, 2014

Area of Science:

Structural biology
Chromatin biology
Molecular genetics

Background:

SWIRM (Swi3, Rsc, Moi2) is a conserved domain in chromatin-associated proteins.
SWIRM proteins are classified into three subfamilies: Swi3, LSD1, and Ada2.
Understanding SWIRM domain structure and function is crucial for chromatin regulation.

Purpose of the Study:

To determine the three-dimensional structure of the SWIRM domain of human MYSM1 (MYb-like, Swirm and Mpn domain-containing protein-1).
To compare the MYSM1 SWIRM structure with other known SWIRM family members.
To investigate the DNA-binding activity of the MYSM1 SWIRM domain and its implications for subfamily divergence.

Main Methods:

X-ray crystallography was used to solve the SWIRM domain structure of MYSM1.
Structural comparisons were performed with known SWIRM domain structures (Swi3, LSD1, Ada2).
Electrophoretic mobility shift assays (EMSAs) were employed to assess DNA-binding activity.

Main Results:

The MYSM1 SWIRM domain adopts a compact HTH-related fold, most closely resembling the Swi3 SWIRM structure.
MYSM1 and LSD1 SWIRM domains lack DNA-binding activity, unlike Ada2alpha and SMARCC2 (Swi3 counterpart).
Structural and functional differences, particularly in the C-terminal helix, explain the varied DNA-binding capabilities within the Swi3/MYSM1 subfamily.

Conclusions:

The SWIRM domain family has diverged into three structural types: Swi3/MYSM1, LSD1, and Ada2.
The Swi3/MYSM1 subfamily exhibits functional divergence, with distinct DNA-binding properties.
MYSM1's SANT domain binds DNA similarly to the c-Myb DNA-binding domain, suggesting distinct functional roles for its domains.