Related Experiment Video
Updated: Jul 15, 2026

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
Published on: June 24, 2019
Structure-dependent relationships between growth temperature of prokaryotes and the amino acid frequency in their
Gisle Saelensminde1, Øyvind Halskau, Ronny Helland
1Computational Biology Unit (CBU), BCCS, University of Bergen, Bergen, Norway. gisle@cbu.uib.no
Abstract:
We studied the amino acid frequency and substitution patterns between homologues of prokaryotic species adapted to temperatures in the range 0-102 degrees C, and found a significant temperature-dependent difference in frequency for many of the amino acids. This was particularly clear when we analysed the surface and core residues separately. The difference between the surface and the core is getting more pronounced in proteins adapted to warmer environments, with a more hydrophobic core, and more charged and long-chained amino acids on the surface of the proteins. We also see that mesophiles have a more similar amino acid composition to psychrophiles than to thermophiles, and that archea appears to have a slightly different pattern of substitutions than bacteria.
More Related Videos
10:31Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
Published on: February 3, 2022
11:51Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
Published on: April 27, 2018
Related Concept Videos
Factors Influencing Microbial Growth: Temperature
Bacterial Protein Maturation
Diversity of Archaea III
Diversity of Archaea IV
Translation in Prokaryotes
Protein Organization