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Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

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ATPase-coupled release control from polyion complex capsules encapsulating muscle proteins.

Kousuke Sugiura¹, Kousaku Ohkawa, Toshihiro Hirai

¹Department of Kansei Engineering, Faculty of Textile Science and Technology, Shinshu University, Japan.

Macromolecular Bioscience

|April 13, 2007

Summary

This study encapsulated muscle proteins into gellan-chitosan capsules, protecting them from degradation. Actomyosin

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Published on: March 10, 2023

Area of Science:

Biomaterials Science
Protein Chemistry
Polymer Science

Background:

Muscle contraction involves the protein complex actomyosin, composed of myosin and actin.
Encapsulation technologies are crucial for protecting sensitive biomolecules.
Polyion complex (PIC) capsules offer potential for controlled release applications.

Purpose of the Study:

To encapsulate actomyosin into gellan-chitosan PIC capsules.
To investigate the functional recovery and stability of encapsulated actomyosin.
To study the release kinetics of small proteins from these capsules and the influence of actomyosin activity.

Main Methods:

Encapsulation of actomyosin (myosin and F-actin) into gellan-chitosan polyion complex (PIC) capsules.
Assay of myosin-ATPase activity to confirm actomyosin complex formation and function.
Protease protection assays to evaluate the stability of encapsulated proteins.
In vitro release studies using myoglobin and cytochrome c under varying ionic strength and pH conditions.
Investigation of release modulation by Mg2+-ATP and encapsulated actomyosin.

Main Results:

Successful encapsulation of actomyosin with approximately 50% myosin-ATPase activity recovery.
Evidence of actomyosin complex formation and Mg2+-ATPase activity stimulation by F-actin.
Encapsulation protected myosin, F-actin, and actomyosin from protease hydrolysis.
Differential release of myoglobin and cytochrome c influenced by ionic strength and pH.
Reduced release of small proteins in the presence of Mg2+-ATP and encapsulated actomyosin, indicating release regulation.

Conclusions:

Gellan-chitosan PIC capsules can successfully encapsulate and protect actomyosin.
The ATPase-coupled sliding motion of actomyosin filaments appears to regulate the pore size of the PIC capsule membranes.
This suggests a novel mechanism for controlled release based on protein activity within the capsule.