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Genomic and proteomic screening of apoptosis mitochondrial regulators for drug target discovery

Gael Lecellier1, Catherine Brenner

  • 1Université de Versailles/SQY, CNRS UMR8159, 45 avenue des Etats-Unis, 78035 Versailles, France.

Current Medicinal Chemistry
|April 14, 2007
PubMed

Insights

Identifying new anticancer drugs and targets is crucial. This review covers screening methods for apoptosis regulators, highlighting a proteomic strategy that found a mitochondrial enzyme controlling cell death.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Apoptosis control is vital for anticancer drug development.
  • Identifying novel therapeutic targets and compounds in apoptosis is a key challenge.
  • The intrinsic apoptotic pathway is a significant focus for cancer research.

Purpose of the Study:

  • To review current screening methodologies for discovering drugs and targets in apoptosis.
  • To focus on screening strategies for the intrinsic apoptotic pathway.
  • To present a proteomic approach for identifying novel apoptosis regulators.

Main Methods:

  • Literature review of screening strategies for apoptosis targets.
  • Proteomic screening approach.
  • Analysis of mitochondrial glutathione-S-transferase and adenine nucleotide translocase interaction.

Main Results:

  • A proteomic strategy successfully identified novel regulators of apoptosis.
  • Mitochondrial glutathione-S-transferase was identified as a key regulator.
  • This enzyme modulates the function of the pro-apoptotic adenine nucleotide translocase pore.

Conclusions:

  • Proteomic screening is effective for discovering apoptosis regulators.
  • Mitochondrial glutathione-S-transferase represents a potential therapeutic target for cancer.
  • Understanding these regulatory mechanisms can advance anticancer drug development.

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