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Related Experiment Video

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Methodology to Metabolically Inactivate Bacteria for Caenorhabditis elegans Research

Methodology to Metabolically Inactivate Bacteria for Caenorhabditis elegans Research

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Class B alkaline stabilization to achieve pathogen inactivation.

Christine L Bean¹, Jacqueline J Hansen, Aaron B Margolin

¹Department of Microbiology, University of New Hampshire, 35 Colovos Rd, ETB Hall Rm. 230, Durham, NH 03824, USA.

International Journal of Environmental Research and Public Health

|April 14, 2007

Summary

Lime stabilization effectively reduces fecal coliforms, Salmonella, and viruses in biosolids within 2 hours. However, Cryptosporidium parvum oocysts persist, suggesting their potential as an indicator for land application safety.

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Area of Science:

Environmental microbiology
Water treatment technologies
Public health

Background:

Lime stabilization is a common method for treating Class B biosolids in the US.
Evaluating pathogen inactivation is crucial for safe land application of biosolids.
Existing methods for monitoring viral pathogens are resource-intensive.

Purpose of the Study:

To assess the efficacy of lime stabilization in inactivating various viral, bacterial, and parasitic pathogens.
To identify potential indicator organisms for monitoring the effectiveness of biosolids treatment.
To explore the use of bacteriophages as indicators for viral inactivation.

Main Methods:

Bench-scale model simulating lime stabilization of biosolids in a water matrix.
Evaluation of pathogen survival including fecal coliforms, Salmonella, adenovirus type 5, rotavirus Wa, bacteriophage MS-2, Cryptosporidium parvum oocysts, Giardia lamblia cysts, and Ascaris lumbricoides ova.
Log reduction calculations for bacterial and viral pathogens.

Main Results:

Fecal coliforms and Salmonella showed a 7-log reduction within 2 hours.
Adenovirus, rotavirus, and bacteriophage MS-2 demonstrated a 4-log reduction in 2 hours.
Giardia lamblia cysts were inactivated, while Ascaris lumbricoides ova and Cryptosporidium parvum oocysts remained viable after 72 hours.

Conclusions:

Lime stabilization effectively inactivates many bacterial and viral pathogens but not Ascaris ova or Cryptosporidium oocysts.
Cryptosporidium parvum oocysts show potential as a more suitable indicator for biosolids land application due to their persistence.
Further research on bacteriophages as indicators could streamline viral monitoring, potentially increasing biosolids land application acceptance.