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Updated: Jul 15, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
Fingerprint analysis of Rhizoma chuanxiong by pressurized capillary electrochromatography and high-performance liquid
Guoxiang Xie1, Aihua Zhao, Peng Li
1School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Abstract:
Pressurized capillary electrochromatography (pCEC) and high-performance liquid chromatography (HPLC) were used simultaneously to establish fingerprints of Rhizoma chuanxiong. Ten batches of Rhizoma chuanxiong collected from different regions in China were used to obtain the characteristic pCEC and HPLC fingerprints using a standardized procedure of sample preparation and analysis. A total of 22 common peaks were isolated within 60 min by pCEC and 16 common peaks by HPLC within 65 min. The fingerprints of Rhizoma chuanxiong were then used to identify the raw herbs from different sources in China. The two proposed methods demonstrated good stability and reproducibility with RSD less than 5% for retention time in pCEC and in HPLC, respectively. Finally, the data from the analyses of 10 batches of Rhizoma chuanxiong by pCEC and HPLC were all processed with similarity analysis with two mathematical methods, correlation coefficient and the included angle cosine. The fingerprints of Rhizoma chuanxiong established with pCEC and HPLC are suitable to identify samples from different sources and can be used to control the quality of raw herbs.
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