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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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RNA Interference

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Related Experiment Video

Updated: Jun 21, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

Regulatory small RNAs in plants.

Cameron Johnson¹, Venkatesan Sundaresan

¹Plant Biology and Plant Sciences, University of California, Davis, CA 95616, USA.

|April 17, 2007

Summary

Small RNAs, including microRNAs (miRNAs), are crucial regulators of gene networks in plants, impacting gene expression, development, and defense. Their discovery has revolutionized our understanding of gene regulation beyond protein-coding genes.

Area of Science:

Molecular Biology
Genetics
Plant Science

Background:

The discovery of microRNAs (miRNAs) has challenged the traditional view of gene regulation, highlighting the importance of small RNA systems.
Small RNAs play diverse roles in eukaryotic cells, including chromatin modification, transposon silencing, and developmental regulation.

Purpose of the Study:

To provide an overview of small RNA functions in plants.
To discuss the distinguishing features of different small RNAs.
To highlight computational and experimental methods for identifying plant small RNAs and their targets.

Main Methods:

Review of existing literature on small RNA biology in plants.
Discussion of computational prediction tools for miRNA and target identification.

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Last Updated: Jun 21, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Examples of experimental validation techniques for small RNA candidates.

Main Results:

Small RNAs regulate gene expression through chromatin states, transposon inhibition, and modulation of transcription factors.
Distinct classes of small RNAs possess unique features and functions.
Computational and experimental approaches are effective for identifying and validating plant small RNAs.

Conclusions:

Small RNAs are integral components of gene regulatory networks in plants.
Understanding small RNA systems is essential for advancing gene regulation studies.
Emerging concepts reveal small RNAs as key modulators of gene expression in multicellular organisms.