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Related Experiment Videos

Single nucleotide polymorphisms for pig identification and parentage exclusion.

G A Rohrer1, B A Freking, D Nonneman

  • 1USDA, ARS, US Meat Animal Research Center, PO Box 166, Spur 18D, Clay Center, NE 68933-0166, USA. gary.rohrer@ars.usda.gov

Animal Genetics
|April 17, 2007
PubMed
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A new panel of 60 single nucleotide polymorphism (SNP) markers was developed for pigs. This panel provides high accuracy for parentage testing and individual identification in commercial swine populations.

Area of Science:

  • Animal Genetics
  • Genomics
  • Molecular Biology

Background:

  • Single nucleotide polymorphisms (SNPs) are crucial for genetic applications in livestock.
  • Accurate SNP allele frequencies are needed for commercial pig populations.
  • Existing SNP marker data is limited for widespread use in swine breeding and diagnostics.

Purpose of the Study:

  • To develop and validate a panel of SNP markers for commercial pig populations.
  • To provide allele frequency data for Duroc, Hampshire, Landrace, and Yorkshire breeds.
  • To assess the utility of SNPs for parentage and individual identification in pigs.

Main Methods:

  • Tested 80 previously mapped SNP markers in 155 boars from four major US pig breeds.
  • Genotyped SNPs using multiplex assays and Sequenom's MassARRAY system.

Related Experiment Videos

  • Selected 60 SNPs with minor allele frequencies >0.15 for the final panel.

    • Main Results:

    • Developed a panel of 60 informative SNP markers with high identity power across and within breeds.
    • Achieved high parentage exclusion probabilities (0.9974 overall, up to 0.9963 within breeds).
    • Demonstrated superior sensitivity for individual identification compared to microsatellite markers.

    Conclusions:

    • The developed SNP panel is highly effective for individual pig identification and parentage testing.
    • This publicly available resource will benefit commercial swine breeding and diagnostic applications.
    • SNP markers offer a powerful alternative to traditional genetic markers in pigs.