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Updated: Aug 15, 2026

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration
Published on: June 7, 2012
Effects of vehicle supplementation on total estradiol absorption from a transdermal estradiol delivery system
R D Smith1, D E Robinson, B Delignieres
1Department of Obstetrics and Gynecology, Georgetown University School of Medicine, Washington, D.C. 20007.
Objective:
To evaluate the effects of vehicle supplementation on serum estradiol (E2) delivery pharmacokinetics from the Ciba-Geigy (Summit, NJ) 0.1-mg Estraderm Patch.
Design:
Postmenopausal women were randomized to a 28-day crossover treatment protocol separated by a 14-day wash out period.
Setting:
Normal human volunteers were studied in an academic research environment.
Patients, Participants:
The subject pool included eight healthy postmenopausal women between 32 and 60 years of age.
Interventions:
In treatment A, a 0.1-mg Estraderm Patch was worn for 7 days; in treatment B, and identical patch was worn into which 0.6 mL of ethanol was injected on day 3 of use.
Main Outcome Measures:
Serum E2 levels were measured in both groups.
Results:
Although E2 absorption showed characteristic interpatient variability, addition of ethanol significantly extended the mean time for serum E2 levels to return to baseline, without increasing peak absorption. The mean extension was 50 hours.
Conclusion:
The addition of ethanol to the Estraderm Patch increased the duration of elevated serum E2 levels measured in menopausal women, thus potentially increasing the effective life span of the transdermal therapeutic system.
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