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Updated: Jul 15, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Multi-grams scale purification of xanthanolides from Xanthium macrocarpum. Centrifugal partition chromatography
Benoit Pinel1, Grégoire Audo, Sabine Mallet
1Laboratoire SONAS EA 921, UFR des Sciences Pharmaceutiques et Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
Abstract:
Plants of the Asteraceae family are known as a source of sesquiterpene lactones with interesting biological activities. The purification of several xanthanolides (xanthathin, 4-epi-xanthanol and 4-epi-isoxanthanol) was realized in one step, directly from the crude chloroformic extract of the leaves of X. macrocarpum by silica gel chromatography and for the first time by liquid/liquid chromatography (counter-current chromatography/centrifugal partition chromatography), using a FCPC 5L (Fast centrifugal partition chromatograph, Kromaton Technologies, Angers, France). Recovery, purity of xanthathin and solvent consumption were improved with the liquid/liquid chromatography compared to solid/liquid chromatography.
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