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Related Experiment Video

Updated: Jul 15, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

A DIGE-based approach to study interacting proteins.

Alex Lyakhovich¹, Francesc Canals, Michael Nosov

¹Group of Mutagenesis, Department of Genetics and Microbiology, Universitat, Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. alex.lyakhovich@uab.es

Journal of Biochemical and Biophysical Methods

|April 17, 2007

Summary

Difference in-gel electrophoresis (DIGE) on native gels offers a novel method for studying protein interactions. This technique successfully demonstrated the interaction between p53 and HDM2 proteins, advancing interactome research.

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Area of Science:

Proteomics
Molecular Biology
Biochemistry

Background:

High-throughput methods for protein identification and characterization are established.
Understanding the protein interactome remains a significant challenge in modern proteomics.
The difference in-gel electrophoresis (DIGE) system shows promise for studying protein interactions.

Purpose of the Study:

To apply the DIGE technique on native gel electrophoresis for studying protein-protein interactions.
To validate the DIGE approach using a known in vitro interaction model.

Main Methods:

Utilized difference in-gel electrophoresis (DIGE) on native gels.
Applied the DIGE technique to study protein-protein interactions.
Used an in vitro interaction model of p53 and HDM2 proteins.
Confirmed interactions using fluorescently labeled immunoprecipitation pellets.

Main Results:

Successfully applied DIGE on native gels to study protein-protein interactions.
Demonstrated the in vitro interaction between p53 and HDM2 proteins.
Validated the DIGE method by comparing results with immunoprecipitation.

Conclusions:

The DIGE technique, when applied to native gel electrophoresis, shows significant potential for investigating protein-protein interactions.
This approach can contribute to the creation of comprehensive interactome maps.
The study provides a proof of principle for using DIGE in interactome research.