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Pattern of epithelial cell cycling in hydra
T W Holstein1, E Hobmayer, C N David
1Department of Zoology, University of Munich, Germany.
Developmental Biology
|December 1, 1991
Summary
In Hydra, epithelial cell proliferation patterns change with feeding, starvation, budding, and regeneration. These shifts are influenced by signals that either stimulate or inhibit mitosis, closely linking cell cycling to morphogenetic events.
Area of Science:
- Developmental Biology
- Cell Biology
- Regenerative Medicine
Background:
- Hydra magnipapillata, a model organism for regeneration and development, exhibits complex cell cycling dynamics.
- Understanding epithelial cell proliferation is crucial for deciphering morphogenetic processes in simple metazoans.
Purpose of the Study:
- To investigate the spatial distribution and regulation of epithelial cell cycling in Hydra magnipapillata.
- To correlate epithelial cell proliferation patterns with feeding, starvation, budding, and regeneration.
Main Methods:
- Utilized a temperature-sensitive mutant strain (sf-1) of Hydra magnipapillata to isolate epithelial cells.
- Employed pulse labeling with 5'-bromo-2'-deoxyuridine (Brdu) to identify S-phase cells.
- Analyzed the spatial distribution of Brdu-labeled cells under various physiological conditions.
Main Results:
- Epithelial cell cycling is broadly and evenly distributed along the body column in basal conditions.
- Feeding and regeneration trigger signaling pathways that transiently inhibit mitosis in gastric tissues.
- Starvation reduces cell cycling in the gastric region, while head tissue and developing buds show increased proliferation.
Conclusions:
- Epithelial cell cycling in Hydra is dynamically regulated by feeding and morphogenetic events.
- Distinct signaling mechanisms stimulate or inhibit mitosis in different regions and during specific developmental processes.
- Cell proliferation patterns are tightly integrated with the organism's overall physiological state and regenerative capacity.