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Effects of exogenous putrescine on murine preimplantation development in vitro
J A Sawicki1, A Impellizeri, T G O'Brien
1Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096.
Developmental Biology
|December 1, 1991
Abstract:
After first demonstrating that murine embryos take up putrescine from the medium in which they are cultured in vitro, fertilized eggs were placed in culture and maintained for 4 days in medium supplemented with varying amounts of putrescine. Their development was monitored each day. While embryos that were cultured in putrescine-supplemented medium developed at the same rate as control embryos, a significantly higher percentage of the putrescine-treated embryos attained the blastocyst stage as compared to the control group.