Related Experiment Video

Updated: Jul 15, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2

Wei Yao1, Daniela Roser, Alwin Köhler

  • 1Biochemie-Zentrum der Universität Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.

Molecular Cell
|April 17, 2007
PubMed

Insights

The Mex67-Mtr2 complex exports mRNA and also functions in ribosomal 60S subunit export. A novel interaction surface on Mex67-Mtr2 binds pre-60S particles and 5S rRNA, crucial for nuclear export.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The Mex67-Mtr2 complex is a key mRNA nuclear export receptor in yeast.
  • Its metazoan homolog, TAP-p15, also functions in mRNA export.

Purpose of the Study:

  • To investigate the role of the yeast Mex67-Mtr2 complex beyond mRNA export.
  • To identify novel interaction partners and functions of Mex67-Mtr2.

Main Methods:

  • Biochemical assays to study protein-protein and protein-RNA interactions.
  • Genetic studies in yeast to analyze nuclear export pathways.
  • Structural analysis of the Mex67-Mtr2 complex.

Main Results:

  • Mex67-Mtr2 mediates the nuclear export of the ribosomal 60S subunit.
  • A new interaction surface on Mex67-Mtr2 binds pre-60S particles and 5S rRNA.
  • Mutations in specific loops of Mex67-Mtr2 disrupt 60S subunit export, causing nuclear accumulation.

Conclusions:

  • The Mex67-Mtr2 complex possesses a dual role in nuclear export, handling both mRNA and ribosomal subunits.
  • A distinct electrostatic interaction surface on Mex67-Mtr2 is responsible for binding 60S preribosomal cargo.

More Related Videos

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells10:24

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Published on: December 17, 2012

Related Concept Videos

Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...