Related Experiment Video
Updated: Jul 15, 2026
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Published on: August 6, 2016
Direct DNA isolation from solid biological sources without pretreatments with proteinase-K and/or homogenization
Jang-Seu Ki1, Ki Byum Chang, Hee June Roh
1Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea. kijs@hanyang.ac.kr
Abstract:
Genomic DNA from solid biomaterials was directly isolated with an automated DNA extractor, which was based on magnetic bead technology with a bore-mediated grinding (BMG) system. The movement of the bore broke down the solid biomaterials, mixed crude lysates thoroughly with reagents to isolate the DNA, and carried the beads to the next step. The BMG system was suitable for the mechanical homogenization of the solid biomaterials and valid as an automated system for purifying the DNA from the solid biomaterials without the need for pretreatment or disruption procedures prior to the application of the solid biomaterials.
Related Concept Videos
01:24DNA Isolation
01:34DNA Isolation
02:35DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...