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Updated: Jul 15, 2026

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Published on: June 24, 2012
Characterisation of a novel recombination event in the norovirus polymerase gene
Allison Waters1, Suzie Coughlan, William W Hall
1Centre for Research in Infectious Diseases (CRID), School of Medical and Medical Sciences, National Virus Reference Laboratory (NVRL), University College Dublin (UCD), Belfield, Dublin 4, Ireland.
Abstract:
This communication describes a novel recombination event in the norovirus genome. Similarity plot analysis of a nucleotide fragment (1003 bp) amplified from a norovirus positive clinical specimen (IrlN05771) identified a previously undescribed recombination point in the 3' region of the polymerase gene (nucleotide position 4889 bp). Nucleotide multiple alignments demonstrated that Irl05N771 shared 78.6% and 94% identity with all other Irish norovirus sequences before and after the recombination point, respectively and confirmed the sequence as a genogroup II/type 4 recombinant. Irl05N771 shared more identity with Asian norovirus sequences. This is the first description of recombination within the norovirus polymerase and highlights the continuous genetic evolution of noroviruses.
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