Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Abnormal permeability pathways in human red blood cells.

J C Ellory1, H C Robinson, J A Browning

  • 1Department of Physiology, Anatomy and Genetics, Sherrington Building, Parks Road, Oxford, OX1 3PT, UK.

Blood Cells, Molecules & Diseases
|April 17, 2007
PubMed
Summary

Abnormal red blood cell (RBC) permeability pathways were investigated in sickle cell disease (SCD) and hereditary stomatocytosis. Findings reveal novel conductance pathways contributing to RBC dehydration and hemolysis in SCD.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Case of Paraplegia from Peripheral Irritation.

The Chicago medical journal·2023
Same author

Case of Wound of the Liver.

The Chicago medical journal·2023
Same author

Successful treatment of a chronic oroantral fistula infected with extensively drug resistant bacteria using long-term oesophageal tube feeding and several non-conventional treatments in a horse.

Australian veterinary journal·2021
Same author

Treatment of moderate grade dog bite wounds using amoxicillin-clavulanic acid with and without enrofloxacin: a randomised non-inferiority trial.

Australian veterinary journal·2021
Same author

Prevalence and spatial distribution of Coxiella burnetii seropositivity in northern Australian beef cattle adjusted for diagnostic test uncertainty.

Preventive veterinary medicine·2021
Same author

Yoda1 and phosphatidylserine exposure in red cells from patients with sickle cell anaemia.

Scientific reports·2020

Area of Science:

  • Physiology
  • Pathology
  • Biophysics

Background:

  • Abnormal red blood cell (RBC) permeability is implicated in various conditions.
  • Sickle cell disease (SCD) involves abnormal hemoglobin (HbS) leading to altered RBC function.
  • Understanding these pathways is crucial for disease management.

Purpose of the Study:

  • To investigate abnormal ion and non-electrolyte conductance pathways in human RBCs.
  • To characterize the P(sickle) conductance in deoxygenated sickle RBCs.
  • To explore the role of mutated band 3 in hereditary stomatocytosis.

Main Methods:

  • Whole-cell patch-clamp recordings on normal and sickle RBCs.
  • Radioisotopic assays to confirm non-electrolyte permeability.
  • Pharmacological inhibition studies using DIDS, Zn(2+), and Gd(3+).

Related Experiment Videos

Main Results:

  • Deoxygenated sickle RBCs exhibit a unique conductance (P(sickle)) permeable to Na+, K+, and Ca2+, and partially inhibited by DIDS and Zn(2+).
  • Gd(3+) significantly reduced conductance in both normal and sickle RBCs.
  • Deoxygenated sickle cells, but not normal RBCs, underwent hemolysis in isosmotic non-electrolyte solutions, inhibited by DIDS, suggesting P(sickle) permeability to non-electrolytes.
  • Mutated band 3 in hereditary stomatocytosis acts as a univalent cation conductance pathway.

Conclusions:

  • P(sickle) is a significant conductance pathway in deoxygenated sickle RBCs, contributing to cell dehydration and potentially hemolysis.
  • P(sickle) may also allow non-electrolyte passage under specific conditions.
  • Mutations in band 3 can create abnormal cation conductance pathways in RBCs, extending the understanding of RBC permeability disorders.