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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
New insights into the Enterococcus faecalis CroRS two-component system obtained using a differential-display random
Yoann Le Breton1, Cécile Muller, Yanick Auffray
1Laboratoire Microbiologie de l'Environnement, EA 956, USC INRA 2017, IRBA, Université de Caen, France. ylebreton@umaryland.edu
Applied and Environmental Microbiology
|April 17, 2007
Summary
The Enterococcus faecalis JH2-2 CroRS system is repressed during stationary phase. Researchers identified a specific CroRS-regulated operon (glnQHMP) and confirmed CroR binds to its promoter.
Area of Science:
- Microbiology
- Molecular Biology
- Bacterial Genetics
Background:
- The Enterococcus faecalis JH2-2 CroRS two-component regulatory system plays a role in bacterial adaptation.
- Understanding gene regulation in bacteria is crucial for controlling their growth and behavior.
Purpose of the Study:
- To investigate the regulation of the CroRS two-component system in Enterococcus faecalis JH2-2.
- To identify operons regulated by the CroRS system and characterize the binding of the CroR regulator.
Main Methods:
- Modified random arbitrarily primed PCR was used to identify regulated operons.
- Gel retardation assays were employed to assess the binding of the CroR regulator to promoter regions.
Main Results:
- The operon encoding the CroRS two-component regulatory system was found to be repressed during the stationary phase.
- A specific CroRS-regulated operon, glnQHMP, was identified.
- The CroR regulator was shown to bind specifically to the glnQHMP promoter.
Conclusions:
- The CroRS two-component system is subject to repression during stationary phase in Enterococcus faecalis.
- The glnQHMP operon is a direct target of the CroRS regulatory system.
- These findings contribute to understanding gene expression control in Enterococcus faecalis.
