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[Study of betacyanin-discolorating enzyme]
Biokhimiia (Moscow, Russia)
|July 1, 1976
Summary
Researchers isolated and purified a beet root enzyme that breaks down betacyanins, causing discoloration. This enzyme, optimal at pH 3.4 and 40°C, contains a metal in its active site.
Area of Science:
- Biochemistry
- Plant Science
Context:
- Betacyanins are pigments found in plants like beets (Beta vulgaris).
- Understanding the enzymes involved in pigment degradation is crucial for food science and plant physiology.
Purpose:
- To isolate and characterize an enzyme responsible for betacyanin discoloration from beet roots.
- To determine the enzyme's optimal activity conditions and properties.
Summary:
- An enzyme was purified 100-fold from beet roots, catalyzing the discoloration and breakdown of betacyanins (betanin, betanidin).
- The enzyme is insoluble and bound to subcellular structures, with optimal activity at pH 3.4 and 40°C.
- Enzyme inhibition studies indicated the presence of a metal in the active site, and the reaction consumed oxygen.
Impact:
- Provides insights into the biochemical pathways of plant pigment degradation.
- Characterization of the enzyme aids in understanding beet quality and potential applications in food processing.