Related Experiment Video
Updated: Jul 15, 2026
09:18
Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
Published on: February 24, 2023
[Synoviocyte cultures from synovial fluid].
A Scanu1, F Oliviero, L Braghetto
1Cattedra e U.O.C. di Reumatologia, Università di Padova, Padova, Italia. anna.scanu@unipd.it
Reumatismo
|April 17, 2007
Summary
Obtaining fibroblast-like synovial cells from synovial fluid (SF) is a simpler alternative to tissue biopsy for rheumatic disease research. These SF-derived cells exhibit similar characteristics and inflammatory responses to those from synovial membranes.
Area of Science:
- Rheumatology
- Cell Biology
- Immunology
Background:
- Rheumatic disease pathogenesis is studied using in vitro cellular models.
- Accessing fresh human synovial tissue for cell culture can be challenging.
Purpose of the Study:
- To develop a simpler method for obtaining fibroblast-like synovial cells from synovial fluid (SF).
- To compare cells derived from SF with those from synovial membranes (SM).
Main Methods:
- Synovial fluid (SF) was collected from patients with rheumatoid arthritis (RA), osteoarthritis (OA), and psoriatic arthritis (PsA).
- Cells were cultured and characterized using immunocytochemistry.
- Inflammatory response to monosodium urate crystals was assessed by measuring nitric oxide (NO) production.
Main Results:
- Adherent fibroblast-like synoviocytes were successfully cultured from SF in most cases.
- SF-derived and SM-derived synoviocytes expressed similar surface antigens (CD90, CD55) and morphology.
- Nitric oxide (NO) levels induced by urate crystals were comparable between SF and SM synoviocytes.
Conclusions:
- Adherent cells from SF possess the phenotype and reactivity of tissue-derived synoviocytes.
- This SF-based method offers a practical alternative for obtaining synoviocytes when synovial tissue is unavailable.
Related Concept Videos
01:16Structural Joints: Synovial Joints
Synovial joints are the most common type of joint in the body. A key structural characteristic for a synovial joint is the presence of a joint cavity. This fluid-filled space is where the articulating surfaces of the bones contact each other. Also, unlike fibrous or cartilaginous joints, the articulating bone surfaces at a synovial joint are not directly connected to each other with fibrous connective tissue or cartilage. This gives the bones of a synovial joint the ability to move smoothly...
01:07Development of the Limb Synovial Joints
Joints form during embryonic development in conjunction with the formation and growth of the associated bones. The embryonic tissue that gives rise to all bones, cartilage, and connective tissues of the body is called mesenchyme.
The mesenchymal stem cells differentiate into chondrocytes that form the hyaline cartilage, and later the cartilaginous model of the bone. This model further transforms into a bone. This process is known as endochondral ossification.
During development, the limbs...
The mesenchymal stem cells differentiate into chondrocytes that form the hyaline cartilage, and later the cartilaginous model of the bone. This model further transforms into a bone. This process is known as endochondral ossification.
During development, the limbs...
01:23Glycosaminoglycans
Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...