A simple competitive protein-binding assay for adenosine-3',5'-monophosphate in plasma and urine

A modified competitive protein-binding assay for the measurement of adenosine-3',5'-monophosphate is described. The procedure allows measurement of adenosine-3',5'-monophosphate in unextracted plasma samples. The mean plasma values in 25 normal, fasting and ambulatory subjects were 22.7 +/- 4.7 pmol/ml (mean +/- SD) (range 13-31 pmol/ml. The mean urinary content was 3.2 +/- 1.0 mumol per g creatinine (mean +/- SD) (n=24).