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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
[The phylogenetic relationship of cultivated isolates of Ganoderma in China inferred from nuclear ribosomal DNA ITS
Chun-li Su1, Chuan-hong Tang, Jing-song Zhang
1The Edible Fungi Institute , the Shanghai Academy of Agricultural Sciences, Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Shanghai 201106, China.
Abstract:
Nuclear ribosomal DNA ITS sequences have been used to investigate phylogenetic relationships between 34 Ganoderma isolates cultivated in China. Five distinct groups were identified: the subgenus Elfvingia, the sect. Phaeonema, and three groups within the sect. Ganoderma. Most of the Ganoderma isolates (85.7%) formed a single group within the sect. Gantoderma. The result indicated clear genetic diversity between the subgenus Elfvingia and the sects Phaeonema and Ganoderma, but a smaller degree of genetic diversity between the three groups placed within the sect. Ganoderma. Analysis of molecular data is a more effective and useful approach for studying the taxonomy of Ganoderma, and for establishing phylogenetic relationships within the genus, compared to methods based on fruiting body morphology.
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