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Updated: Jul 15, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Factor VII polymorphisms influence the plasma response to diets with different fat content, in a healthy Caucasian
Rafael Angel Fernández de la Puebla1, Pablo Pérez-Martínez, José Carmona
1Reina Sofia University Hospital, Lipids and Atherosclerosis Research Unit, University of Cordoba, Ciber Fisiopatologia Obesidad y Nutricion, Instituto Salud Carlos III, Cordoba, Spain.
Unlabelled:
To determine the influence of the factor VII gene polymorphisms, R353Q and 5'F7, on factor VII Ag plasma levels after the consumption of diets with different fat contents.
Methods:
59 healthy individuals (42 RR, 16 RQ, 1 QQ at the R353Q and 46 A1A1, 13 A1A2 at the 5'F7) consumed 3 diets during 4-weeks each: a Saturated diet (SAT) enriched in saturated fatty acid (SFA) (38% fat, 20% SFA), followed by a carbohydrate (CHO)-rich diet (30% fat, 55% CHO) or a Mediterranean diet (MEDIT) enriched in monounsaturated fatty acid (MUFA) (38% fat, 22% MUFA) following a randomized crossover design. Plasma lipids and FVII Ag plasma levels were determined at the end of each dietary period.
Results:
After a SAT diet, RR homozygotes had greater concentrations of FVII Ag compared with MEDIT and CHO diets than did carriers of the minority Q allele (82.76 +/- 1.3 vs. 75.02 +/- 2.4, p = 0.001). The 5'F7 polymorphism behaved in a similar fashion (A1A1 81.98 +/- 1.4 vs. A1A2 75.37 +/- 2.4, p = 0.026).
Conclusions:
Our data show that carriers of the RR and/or A1A1 genotype present higher FVII Ag levels after the consumption of a SAT diet compared with the MEDIT and CHO rich diets.
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