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Updated: Jul 15, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Published on: December 8, 2023
[Protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis]
Zheng-Ling Shang1, Lang Bao, Su-Xia Yao
1Department of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis.
Methods:
By PCR technique, the complete open-reading frame sequences of Rv1246c and Rv1247c gene were amplified from the M. tuberculosis H37Rv genomic DNA as template. The PCR-amplified cDNAs of Rv1247c and Rv1246c gene were subcloned into pGBKT7 and pGADT7-Rec vector respectively for constructing recombinant plasmids pGBKT7-Rv1247c and pGADT7-Rv1246c. After verified by restriction endonuclease digestion and DNA sequence determination, the recombinant vectors were used to transform the yeast cell AH109 by lithium acetate method.
Results:
The yeast cells co-transformed with pGBKT7-Rv1247c and pGADT7-Rv1246c grew on SD/-Ade/-His/-Leu/-Trp plates, and the beta-galactosidase activity assays showed the positive signal.
Conclusion:
The hypothetical protein Rv1246c and Rv1247c could interact with each other in yeast cells.
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