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Application of capillary zone electrophoresis to the characterization of multiple antigen peptides
A Pessi1, E Bianchi, L Chiappinelli
1Peptide Synthesis Unit, Sclavo SpA, Rome, Italy.
Journal of Chromatography
|September 20, 1991
Abstract:
Capillary zone electrophoresis (CZE) was applied to the analysis of a recently described class of synthetic branched peptides, multiple antigen peptides (MAPs). In comparison with high-performance liquid chromatography (HPLC), CZE showed superior resolution of minor impurities in samples of MAPs obtained by preparative chromatography. MAPs that differed only in the substitution of uncharged residues (Ala-Ser) could be separated by the addition of organic solvents (acetonitrile, methanol, ethanol) to the aqueous background electrolyte.