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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 15, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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Babesia bovis: the development of an expression oligonucleotide microarray.

Audrey O T Lau1, David L Tibbals, Terry F McElwain

  • 1Program in Microbial Genomics, Department of Veterinary Microbiology & Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, United States. laua@vetmed.wsu.edu

Experimental Parasitology
|April 20, 2007
PubMed
Summary

This study presents a Babesia bovis gene expression profile for the intraerythrocytic stage using a custom microarray. This profile aids in identifying potential vaccine antigens by highlighting highly expressed genes.

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Area of Science:

  • Parasitology
  • Molecular Biology
  • Vaccinology

Background:

  • Babesia bovis causes significant economic losses in cattle.
  • Identifying stage-specific gene expression is crucial for developing effective vaccines.
  • Understanding gene function during the intraerythrocytic stage is key to parasite control.

Purpose of the Study:

  • To generate and validate a custom microarray for Babesia bovis gene expression analysis.
  • To establish a stage-specific expression profile of intraerythrocytic Babesia bovis.
  • To identify candidate vaccine antigens based on gene expression levels.

Main Methods:

  • Development and validation of a custom high-density oligonucleotide microarray for Babesia bovis.
  • Analysis of gene expression in in vitro cultured intraerythrocytic stage parasites.
  • Distinguishing parasite gene expression from host (bovine) message.
  • Quantitative real-time PCR (qRT-PCR) for validating microarray results.

Main Results:

  • A comprehensive gene expression profile for over 1000 Babesia bovis genes during the intraerythrocytic stage was established.
  • Gene expression rankings were validated using qRT-PCR on selected genes.
  • Microarray results were consistent with previous expression sequence tag (EST) studies.
  • The custom microarray proved suitable for Babesia bovis gene expression analysis.

Conclusions:

  • The developed microarray is a reliable tool for studying Babesia bovis gene expression.
  • The presented expression profile provides valuable insights into parasite biology during the intraerythrocytic stage.
  • This resource facilitates the identification of novel vaccine candidates against Babesia bovis infections.