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Updated: Jul 15, 2026
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Published on: February 22, 2017
[Analysis on bioactivity of HIV-1 integrase by ELISA method]
Wei-Hong Feng1, Jian-Song Huang, Jin-Biao Zhan
1Department of Biochemistry and Molecular Biology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
Objective:
To develop an ELISA-based method for analyzing biologic activities of HIV-1 integrase and for high throughput screening of integrase inhibitors.
Methods:
After expression, renaturation and purification of integrase, the bioactivity of integrase and the inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and chemiluminescent substrates.
Result:
(1) The specific activity of the purified integrase was 54.92 units/mg of protein. (2)IC(50) (concentration causing 50% inhibition of integrase) of luffin-a was (0.63 +/- 0.026) micromol/L.
Conclusion:
The non-radioactive assay can be used for analysis of bioactivities and high throughput screening of inhibitors of HIV-1 integrase.
